DNA

Part:BBa_K2018043:Design

Designed by: Rune Øbo   Group: iGEM16_SDU-Denmark   (2016-10-07)


MaSp1 CD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 115


Design Notes

might not the best design with an overhang of only 4bp, needed more to be room temputure ligated optimal.


Source

Nephilia clavipes

References